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生物技术研究团队研究生在SCI 期刊Acta Physiol Plant发表论文
文章作者: 发布时间: 2013-07-04 访问次数: 1003

  Molecular cloning and expression analysis of a squalene synthase gene from a medicinal plant, Euphorbia pekinensis Rupr.

Zhujun Zheng • Xiaoying Cao • Changgen Li •Yongqiang Chen • Bo Yuan • Yan Xu •Jihong Jiang 

 

Source

The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.

 

 

Abstract Euphorbia pekinensis Rupr., which is also known as a medicinal plant, produces a large amount of alkaloids, phytosterols and triterpenes. In this study, we reported on the cDNA cloning and characterization of a novel squalene synthase (SQS) from E. pekinensis. Squalene synthase catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to produce squalene (SQ), the first committed precursor for sterol and triterpene biosynthesis.The full length cDNA named EpSQS (Genbank

Accession Number JX509735) contained 1,614 bp with an open reading frame of 1,236 bp encoding a polypeptide of 411 amino acids. The deduced amino acid sequence of the EpSQS named EpSQS exhibited a high homology with other plant SQSs, and contained a single domain surrounded by helices. Phylogenetic analysis showed that EpSQS belonged to the plant SQS kingdom. Tissue expression analysis revealed that EpSQS expressed strongly in roots, weakly in stems and leaves, implying that EpSQS was a constitutive expression gene. The recombinant protein was expressed in Escherichia coli and detected by SDS-PAGE and western blot. The high performance liquid chromatography (HPLC) analysis showed that EpSQS could catalyze the reaction from farnesyl diphosphate (FPP) to squalene.

Keywords Gene cloning Euphorbia pekinensis Rupr.  Squalene synthase Recombinant protein  Real-Time RT-PCT

 

 

 

 全文连接:

 

10.1007_s11738-013-1333-17.pdf

 

作者简介:

     郑竹君,男,系江苏师范大学江苏省药用植物生物技术重点实验室2013届硕士研究生,研究方向为:药用植物分子生物学技术

 

 acta physiologiae plantarum杂志介绍

  该杂志主要以植物生理为主要研究内容,每季度出版,杂志由Polish Academy of Science出版,ISSN号:0137-5881 该杂志2013年影响因子达1.693

 

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