管理登录 加入收藏 设为首页 联系我们 旧版入口
Nocardioides panzhihuaensis sp. nov., a novel endophytic actinomycete isolated from medicinal plant Jatropha curcas L.
文章作者: 发布时间: 2013-07-04 访问次数: 200

2012 Aug;102(2):353-60. Epub 2012 May 3.

Nocardioides panzhihuaensis sp. nov., a novel endophytic actinomycete isolated from medicinal plant Jatropha curcas L.

Source

The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, People's Republic of China.

Abstract

A novel Gram-positive, aerobic, rod-shaped and mycelia-producing bacterial strain, designated KLBMP 1050(T), was isolated from the stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate KLBMP 1050(T) belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides albus KCTC 9186(T) (99.38 %) and Nocardioides luteus KCTC 9575(T) (99.03 %). However, the DNA-DNA relatedness of isolate KLBMP 1050(T) to these two type strains were 37.5 ± 3.5 and 33 ± 2.3 %, respectively. Strain KLBMP 1050(T) grew at the pH range 6-11, temperature range 10-32 °C and with 0-12 % NaCl. The physiological properties of strain KLBMP 1050(T) differ from those of N. albus KCTC 9186(T) and N. luteus KCTC 9575(T). The cell-wall peptidoglycan contained LL: -diaminopimelic acid and MK-8(H(4)) was the major respiratory quinone. The predominant cellular fatty acid of strain KLBMP 1050(T) was iso-C(16:0) (23.3 %). The total DNA G+C content was 70.1 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain KLBMP 1050(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides panzhihuaensis sp. nov. is proposed. The type strain is KLBMP 1050(T) (= KCTC 19888(T) = NBRC 108680(T)).

Nocardioides panzhihuaensis.pdf

江苏师范大学 |  行政办公网 |  科技处 |  生命科学学院 |  化学化工学院
中华人民共和国教育部 |  中华人民共和国科技部 |  国家自然科学基金委员会 |  江苏省科学技术厅
地址:江苏省徐州市铜山新区上海路101号 邮编:221116 邮箱:klbmp@jsnu.edu.cn 电话:0516-83403515 传真:0516-83403515
『江苏省药用植物生物技术重点实验室』整站版权所有©2010 网站制作:李健 COPYRIGHT KLBMP. ALL RIGHTS RESERVED, 2010. DESIGNER:LJ.